A company hosts a software solution on Amazon EC2 instances within a cluster placement group. The solution's UI consists of a single HTML page sized at 1,024 bytes, while the software processes files larger than 1,024 MB. Files are shared over the network to clients upon request, with the Don't Fragment flag enabled. The EC2 instances' elastic network interfaces are configured with jumbo frames.

The UI remains accessible from all permitted source IP addresses, whether they are within a VPC, on the internet, or on-premises. However, clients occasionally fail to receive requested files due to transmission issues between the software and the clients.

Which two options could be potential root causes of these transmission failures?